diff --git a/tox.ini b/tox.ini
index 2776527ee..435ac629d 100644
--- a/tox.ini
+++ b/tox.ini
@@ -596,7 +596,6 @@ deps =
   flaky==3.7.0
   remoulade>=0.50
   mysqlclient~=2.1.1
-  pyyaml==6.0
 
 changedir =
   tests/opentelemetry-docker-tests/tests