diff --git a/core/src/components/item/test/a11y/item.e2e.ts b/core/src/components/item/test/a11y/item.e2e.ts
index db936bf64b..20536beb71 100644
--- a/core/src/components/item/test/a11y/item.e2e.ts
+++ b/core/src/components/item/test/a11y/item.e2e.ts
@@ -2,6 +2,35 @@ import AxeBuilder from '@axe-core/playwright';
 import { expect } from '@playwright/test';
 import { configs, test } from '@utils/test/playwright';
 
+configs({ directions: ['ltr'], palettes: ['dark'] }).forEach(({ config, screenshot, title }) => {
+  test.describe(title('item: dark palette'), () => {
+    /**
+     * This test was originally created to ensure the item border has sufficient
+     * contrast. We don't use an Axe test here because Axe not warn about color
+     * contrast on the item borders.
+     */
+    test('borders should have sufficient contrast', async ({ page }) => {
+      test.info().annotations.push({
+        type: 'issue',
+        description: 'https://github.com/ionic-team/ionic-framework/issues/29386',
+      });
+      await page.setContent(
+        `
+        <ion-list>
+          <ion-item>Item</ion-item>
+          <ion-item>Item</ion-item>
+        </ion-list>
+      `,
+        config
+      );
+
+      const list = page.locator('ion-list');
+
+      await expect(list).toHaveScreenshot(screenshot(`item-dark`));
+    });
+  });
+});
+
 configs({ directions: ['ltr'] }).forEach(({ config, screenshot, title }) => {
   test.describe(title('item: axe'), () => {
     test('should not have accessibility violations', async ({ page }) => {
diff --git a/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-ios-ltr-dark-Mobile-Chrome-linux.png b/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-ios-ltr-dark-Mobile-Chrome-linux.png
new file mode 100644
index 0000000000..e5129d4a01
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diff --git a/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-ios-ltr-dark-Mobile-Firefox-linux.png b/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-ios-ltr-dark-Mobile-Firefox-linux.png
new file mode 100644
index 0000000000..c75f1c1f0e
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diff --git a/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-ios-ltr-dark-Mobile-Safari-linux.png b/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-ios-ltr-dark-Mobile-Safari-linux.png
new file mode 100644
index 0000000000..ddd58da378
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diff --git a/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-md-ltr-dark-Mobile-Chrome-linux.png b/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-md-ltr-dark-Mobile-Chrome-linux.png
new file mode 100644
index 0000000000..ea2410f108
Binary files /dev/null and b/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-md-ltr-dark-Mobile-Chrome-linux.png differ
diff --git a/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-md-ltr-dark-Mobile-Firefox-linux.png b/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-md-ltr-dark-Mobile-Firefox-linux.png
new file mode 100644
index 0000000000..d500d6cbfa
Binary files /dev/null and b/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-md-ltr-dark-Mobile-Firefox-linux.png differ
diff --git a/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-md-ltr-dark-Mobile-Safari-linux.png b/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-md-ltr-dark-Mobile-Safari-linux.png
new file mode 100644
index 0000000000..e22cd97879
Binary files /dev/null and b/core/src/components/item/test/a11y/item.e2e.ts-snapshots/item-dark-md-ltr-dark-Mobile-Safari-linux.png differ
diff --git a/core/src/css/palettes/dark.scss b/core/src/css/palettes/dark.scss
index 87455bdbec..0c5ef08dd9 100644
--- a/core/src/css/palettes/dark.scss
+++ b/core/src/css/palettes/dark.scss
@@ -142,7 +142,6 @@ $colors:  (
     --ion-background-color-rgb: 18, 18, 18;
     --ion-text-color: #ffffff;
     --ion-text-color-rgb: 255, 255, 255;
-    --ion-border-color: #222222;
     --ion-background-color-step-50: #1e1e1e;
     --ion-background-color-step-100: #2a2a2a;
     --ion-background-color-step-150: #363636;