diff --git a/core/package-lock.json b/core/package-lock.json
index 9f5c5a82d5..62be248676 100644
--- a/core/package-lock.json
+++ b/core/package-lock.json
@@ -14,6 +14,7 @@
         "tslib": "^2.1.0"
       },
       "devDependencies": {
+        "@axe-core/puppeteer": "^4.1.1",
         "@jest/core": "^26.6.3",
         "@rollup/plugin-node-resolve": "^8.4.0",
         "@rollup/plugin-virtual": "^2.0.3",
@@ -43,6 +44,21 @@
         "typescript": "^4.0.5"
       }
     },
+    "node_modules/@axe-core/puppeteer": {
+      "version": "4.1.1",
+      "resolved": "https://registry.npmjs.org/@axe-core/puppeteer/-/puppeteer-4.1.1.tgz",
+      "integrity": "sha512-Ao9N7HL//s26hdasx3Ba18tlJgxpoO+1SmIN6eSx5vC50dqYhiRU0xp6wBKWqzo10u1jpzl/s4RFsOAuolFMBA==",
+      "dev": true,
+      "dependencies": {
+        "axe-core": "^4.1.1"
+      },
+      "engines": {
+        "node": ">=6.4.0"
+      },
+      "peerDependencies": {
+        "puppeteer": ">=1.10.0 < 6"
+      }
+    },
     "node_modules/@babel/code-frame": {
       "version": "7.10.1",
       "resolved": "https://registry.npmjs.org/@babel/code-frame/-/code-frame-7.10.1.tgz",
@@ -2029,6 +2045,15 @@
       "integrity": "sha512-zg7Hz2k5lI8kb7U32998pRRFin7zJlkfezGJjUc2heaD4Pw2wObakCDVzkKztTm/Ln7eiVvYsjqak0Ed4LkMDA==",
       "dev": true
     },
+    "node_modules/axe-core": {
+      "version": "4.1.4",
+      "resolved": "https://registry.npmjs.org/axe-core/-/axe-core-4.1.4.tgz",
+      "integrity": "sha512-Pdgfv6iP0gNx9ejRGa3zE7Xgkj/iclXqLfe7BnatdZz0QnLZ3jrRHUVH8wNSdN68w05Sk3ShGTb3ydktMTooig==",
+      "dev": true,
+      "engines": {
+        "node": ">=4"
+      }
+    },
     "node_modules/babel-plugin-istanbul": {
       "version": "6.0.0",
       "resolved": "https://registry.npmjs.org/babel-plugin-istanbul/-/babel-plugin-istanbul-6.0.0.tgz",
@@ -13802,6 +13827,15 @@
     }
   },
   "dependencies": {
+    "@axe-core/puppeteer": {
+      "version": "4.1.1",
+      "resolved": "https://registry.npmjs.org/@axe-core/puppeteer/-/puppeteer-4.1.1.tgz",
+      "integrity": "sha512-Ao9N7HL//s26hdasx3Ba18tlJgxpoO+1SmIN6eSx5vC50dqYhiRU0xp6wBKWqzo10u1jpzl/s4RFsOAuolFMBA==",
+      "dev": true,
+      "requires": {
+        "axe-core": "^4.1.1"
+      }
+    },
     "@babel/code-frame": {
       "version": "7.10.1",
       "resolved": "https://registry.npmjs.org/@babel/code-frame/-/code-frame-7.10.1.tgz",
@@ -15481,6 +15515,12 @@
       "integrity": "sha512-zg7Hz2k5lI8kb7U32998pRRFin7zJlkfezGJjUc2heaD4Pw2wObakCDVzkKztTm/Ln7eiVvYsjqak0Ed4LkMDA==",
       "dev": true
     },
+    "axe-core": {
+      "version": "4.1.4",
+      "resolved": "https://registry.npmjs.org/axe-core/-/axe-core-4.1.4.tgz",
+      "integrity": "sha512-Pdgfv6iP0gNx9ejRGa3zE7Xgkj/iclXqLfe7BnatdZz0QnLZ3jrRHUVH8wNSdN68w05Sk3ShGTb3ydktMTooig==",
+      "dev": true
+    },
     "babel-plugin-istanbul": {
       "version": "6.0.0",
       "resolved": "https://registry.npmjs.org/babel-plugin-istanbul/-/babel-plugin-istanbul-6.0.0.tgz",
diff --git a/core/package.json b/core/package.json
index 94fa265a07..61d18545f2 100644
--- a/core/package.json
+++ b/core/package.json
@@ -36,6 +36,7 @@
     "tslib": "^2.1.0"
   },
   "devDependencies": {
+    "@axe-core/puppeteer": "^4.1.1",
     "@jest/core": "^26.6.3",
     "@rollup/plugin-node-resolve": "^8.4.0",
     "@rollup/plugin-virtual": "^2.0.3",
diff --git a/core/src/components/segment-button/segment-button.scss b/core/src/components/segment-button/segment-button.scss
index 2c6d1a1ef2..7342a9337a 100644
--- a/core/src/components/segment-button/segment-button.scss
+++ b/core/src/components/segment-button/segment-button.scss
@@ -166,6 +166,10 @@
   }
 }
 
+:host(:focus) {
+  outline: none;
+}
+
 // Segment Button: Hover
 // --------------------------------------------------
 
diff --git a/core/src/components/segment-button/segment-button.tsx b/core/src/components/segment-button/segment-button.tsx
index a030f0c3d9..92468d0977 100644
--- a/core/src/components/segment-button/segment-button.tsx
+++ b/core/src/components/segment-button/segment-button.tsx
@@ -86,13 +86,28 @@ export class SegmentButton implements ComponentInterface, ButtonInterface {
     }
   }
 
+  private get tabIndex() {
+    if (this.disabled) { return -1; }
+
+    const hasTabIndex = this.el.hasAttribute('tabindex');
+
+    if (hasTabIndex) {
+      return this.el.getAttribute('tabindex');
+    }
+
+    return 0;
+  }
+
   render() {
-    const { checked, type, disabled, hasIcon, hasLabel, layout, segmentEl } = this;
+    const { checked, type, disabled, hasIcon, hasLabel, layout, segmentEl, tabIndex } = this;
     const mode = getIonMode(this);
     const hasSegmentColor = () => segmentEl !== null && segmentEl.color !== undefined;
     return (
       <Host
+        role="tab"
+        aria-selected={checked ? 'true' : 'false'}
         aria-disabled={disabled ? 'true' : null}
+        tabIndex={tabIndex}
         class={{
           [mode]: true,
           'in-toolbar': hostContext('ion-toolbar', this.el),
@@ -113,7 +128,7 @@ export class SegmentButton implements ComponentInterface, ButtonInterface {
       >
         <button
           type={type}
-          aria-pressed={checked ? 'true' : 'false'}
+          tabIndex={-1}
           class="button-native"
           part="native"
           disabled={disabled}
diff --git a/core/src/components/segment/segment.tsx b/core/src/components/segment/segment.tsx
index e482cb6ca6..9c4bb1b800 100644
--- a/core/src/components/segment/segment.tsx
+++ b/core/src/components/segment/segment.tsx
@@ -425,6 +425,7 @@ export class Segment implements ComponentInterface {
     const mode = getIonMode(this);
     return (
       <Host
+        role="tablist"
         onClick={this.onClick}
         class={createColorClasses(this.color, {
           [mode]: true,
diff --git a/core/src/components/segment/test/a11y/e2e.ts b/core/src/components/segment/test/a11y/e2e.ts
new file mode 100644
index 0000000000..4c425f42f7
--- /dev/null
+++ b/core/src/components/segment/test/a11y/e2e.ts
@@ -0,0 +1,11 @@
+import { newE2EPage } from '@stencil/core/testing';
+import { AxePuppeteer } from '@axe-core/puppeteer';
+
+test('segment: axe', async () => {
+  const page = await newE2EPage({
+    url: '/src/components/segment/test/a11y?ionic:_testing=true'
+  });
+
+  const results = await new AxePuppeteer(page).analyze();
+  expect(results.violations.length).toEqual(0);
+});
diff --git a/core/src/components/segment/test/a11y/index.html b/core/src/components/segment/test/a11y/index.html
new file mode 100644
index 0000000000..0f4956255e
--- /dev/null
+++ b/core/src/components/segment/test/a11y/index.html
@@ -0,0 +1,31 @@
+<!DOCTYPE html>
+<html lang="en" dir="ltr">
+  <head>
+    <meta charset="UTF-8">
+    <title>Segment - a11y</title>
+    <meta name="viewport"
+      content="width=device-width, initial-scale=1.0, minimum-scale=1.0">
+    <link href="../../../../../css/core.css" rel="stylesheet">
+    <link href="../../../../../scripts/testing/styles.css" rel="stylesheet">
+    <script src="../../../../../scripts/testing/scripts.js"></script>
+    <script nomodule src="../../../../../dist/ionic/ionic.js"></script>
+    <script type="module" src="../../../../../dist/ionic/ionic.esm.js"></script>
+  </head>
+
+  <body>
+    <main>
+      <h1>Segment</h1>
+      <ion-segment aria-label="Tab Options" color="dark" value="reading-list">
+        <ion-segment-button value="bookmarks">
+          <ion-label>Bookmarks</ion-label>
+        </ion-segment-button>
+        <ion-segment-button value="reading-list">
+          <ion-label>Reading List</ion-label>
+        </ion-segment-button>
+        <ion-segment-button value="shared-links">
+          <ion-label>Shared Links</ion-label>
+        </ion-segment-button>
+      </ion-segment>
+    </main>
+  </body>
+</html>
diff --git a/core/src/components/segment/test/a11y/screen-readers.md b/core/src/components/segment/test/a11y/screen-readers.md
new file mode 100644
index 0000000000..dfe3a221e3
--- /dev/null
+++ b/core/src/components/segment/test/a11y/screen-readers.md
@@ -0,0 +1,24 @@
+"native" refers to this sample: https://w3c.github.io/aria-practices/examples/tabs/tabs-2/tabs.html
+
+### Tabbing to Segment Button
+
+|                          | native                                           | Ionic                                            |
+| ------------------------ | ------------------------------------------------ | ------------------------------------------------ |
+| VoiceOver macOS - Chrome | BOOKMARKS, tab, 1 of 3, Tab Options, tab group   | BOOKMARKS, tab, 1 of 3, Tab Options, tab group   |
+| VoiceOver macOS - Safari | BOOKMARKS, tab, 1 of 3, Tab Options, tab group   | BOOKMARKS, tab, 1 of 3, Tab Options, tab group   |
+| VoiceOver iOS            | Bookmarks, tab                                   | Bookmarks, tab                                   |
+| Android TalkBack         | Bookmarks, tab                                   | Bookmarks, tab                                   |
+| Windows NVDA             | Tab Options, tab control, BOOKMARKS, tab, 1 of 3 | Tab Options, tab control, BOOKMARKS, tab, 1 of 3 |
+
+### Selecting Segment Button
+
+|                          | native                                                   | Ionic                    |
+| ------------------------ | -------------------------------------------------------- | ------------------------ |
+| VoiceOver macOS - Chrome | BOOKMARKS, selected, tab, 1 of 3, Tab Options, tab group | BOOKMARKS, selected, tab, 1 of 3, Tab Options, tab group |
+| VoiceOver macOS - Safari | BOOKMARKS, selected, tab, 1 of 3, Tab Options, tab group | BOOKMARKS, selected, tab, 1 of 3, Tab Options, tab group |
+| VoiceOver iOS            | selected, Bookmarks, tab                                 | selected, Bookmarks, tab |
+| Android TalkBack         | selected                                                 | selected                       |
+| Windows NVDA             | BOOKMARKS, tab, 1 of 3, selected                         | BOOKMARKS, tab, 1 of 3, selected                       |
+
+Note: The `aria-label` for tablist is typically only read on the first interaction.
+