diff --git a/core/src/components/radio-group/radio-group.tsx b/core/src/components/radio-group/radio-group.tsx
index 30deb9add1..8f9ed6a693 100644
--- a/core/src/components/radio-group/radio-group.tsx
+++ b/core/src/components/radio-group/radio-group.tsx
@@ -141,10 +141,11 @@ export class RadioGroup implements ComponentInterface {
       }
 
       // Update the radio group value when a user presses the
-      // space bar on top of a selected radio (only applies
-      // to radios in a select popover)
+      // space bar on top of a selected radio
       if (['Space'].includes(ev.code)) {
-        this.value = current.value;
+        this.value = (this.allowEmptySelection && this.value !== undefined)
+          ? undefined
+          : current.value;
 
         // Prevent browsers from jumping
         // to the bottom of the screen
diff --git a/core/src/components/radio-group/test/radio-group.e2e.ts b/core/src/components/radio-group/test/radio-group.e2e.ts
new file mode 100644
index 0000000000..7794c85522
--- /dev/null
+++ b/core/src/components/radio-group/test/radio-group.e2e.ts
@@ -0,0 +1,88 @@
+import { newE2EPage } from '@stencil/core/testing';
+
+/**
+ * @param page the E2E page that contains the radio button
+ * @param radioButtonId the id of the radio button to focus
+ * @returns the checked property of the focused radio button
+ */
+const selectRadio = async (page, radioButtonId: string, selectionMethod: 'keyboard' | 'mouse'): Promise<boolean> => {
+  const selector = `ion-radio#${radioButtonId}`;
+  if (selectionMethod === 'keyboard') {
+    await page.focus(selector);
+    await page.keyboard.press('Space');
+  } else if (selectionMethod === 'mouse') {
+    await page.click(selector);
+  }
+  
+  await page.waitForChanges();
+
+  const radioGroup = await page.find(`ion-radio#${radioButtonId} >>> input`);
+  const checked = await radioGroup.getProperty('checked');
+  return checked;
+}
+
+describe('radio-group', () => {
+  it('Spacebar should not deselect without allowEmptySelection', async () => {
+    const page = await newE2EPage();
+    await page.setContent(`
+      <ion-radio-group value="one" allow-empty-selection="false">
+        <ion-item>
+          <ion-label>One</ion-label>
+          <ion-radio id="one" value="one"></ion-radio>
+        </ion-item>
+      </ion-radio-group>
+    `);
+
+    const checked = await selectRadio(page, 'one', 'keyboard');
+    
+    expect(checked).toBe(true);
+  });
+
+  it('Spacebar should deselect with allowEmptySelection', async () => {
+    const page = await newE2EPage();
+    await page.setContent(`
+      <ion-radio-group value="one" allow-empty-selection="true">
+        <ion-item>
+          <ion-label>One</ion-label>
+          <ion-radio id="one" value="one"></ion-radio>
+        </ion-item>
+      </ion-radio-group>
+    `);
+
+    const checked = await selectRadio(page, 'one', 'keyboard');
+
+    expect(checked).toBe(false);
+  });
+
+  it('Click should not deselect without allowEmptySelection', async () => {
+    const page = await newE2EPage();
+    await page.setContent(`
+      <ion-radio-group value="one" allow-empty-selection="false">
+        <ion-item>
+          <ion-label>One</ion-label>
+          <ion-radio id="one" value="one"></ion-radio>
+        </ion-item>
+      </ion-radio-group>
+    `);
+
+    const checked = await selectRadio(page, 'one', 'mouse');
+    
+    expect(checked).toBe(true);
+  });
+
+  it('Click should deselect with allowEmptySelection', async () => {
+    const page = await newE2EPage();
+    await page.setContent(`
+      <ion-radio-group value="one" allow-empty-selection="true">
+        <ion-item>
+          <ion-label>One</ion-label>
+          <ion-radio id="one" value="one"></ion-radio>
+        </ion-item>
+      </ion-radio-group>
+    `);
+
+    const checked = await selectRadio(page, 'one', 'mouse');
+
+    expect(checked).toBe(false);
+  });
+});