diff --git a/core/src/components/item-sliding/item-sliding.tsx b/core/src/components/item-sliding/item-sliding.tsx
index 1c2a7c7548..1aefe5b504 100644
--- a/core/src/components/item-sliding/item-sliding.tsx
+++ b/core/src/components/item-sliding/item-sliding.tsx
@@ -127,7 +127,16 @@ export class ItemSliding implements ComponentInterface {
    */
   @Method()
   async open(side: Side | undefined) {
-    if (this.item === null) {
+    /**
+     * It is possible for the item to be added to the DOM
+     * after the item-sliding component was created. As a result,
+     * if this.item is null, then we should attempt to
+     * query for the ion-item again.
+     * However, if the item is already defined then
+     * we do not query for it again.
+     */
+    const item = (this.item = this.item ?? this.el.querySelector('ion-item'));
+    if (item === null) {
       return;
     }
 
diff --git a/core/src/components/item-sliding/test/async/index.html b/core/src/components/item-sliding/test/async/index.html
new file mode 100644
index 0000000000..401e37dc96
--- /dev/null
+++ b/core/src/components/item-sliding/test/async/index.html
@@ -0,0 +1,49 @@
+<!DOCTYPE html>
+<html lang="en" dir="ltr">
+  <head>
+    <meta charset="UTF-8" />
+    <title>Item Sliding - Async</title>
+    <meta
+      name="viewport"
+      content="width=device-width, initial-scale=1.0, minimum-scale=1.0, maximum-scale=1.0, user-scalable=no, viewport-fit=cover"
+    />
+    <link href="../../../../../css/ionic.bundle.css" rel="stylesheet" />
+    <link href="../../../../../scripts/testing/styles.css" rel="stylesheet" />
+    <script src="../../../../../scripts/testing/scripts.js"></script>
+    <script nomodule src="../../../../../dist/ionic/ionic.js"></script>
+    <script type="module" src="../../../../../dist/ionic/ionic.esm.js"></script>
+  </head>
+
+  <body>
+    <ion-app>
+      <ion-header>
+        <ion-toolbar>
+          <ion-title>Item Sliding - Basic</ion-title>
+        </ion-toolbar>
+      </ion-header>
+
+      <ion-content>
+        <ion-list>
+          <ion-item-sliding>
+            <ion-item-options side="end">
+              <ion-item-option>Option</ion-item-option>
+            </ion-item-options>
+          </ion-item-sliding>
+        </ion-list>
+
+        <script>
+          const itemSliding = document.querySelector('ion-item-sliding');
+          setTimeout(() => {
+            const item = document.createElement('ion-item');
+            item.innerText = 'Item Sliding Option';
+            itemSliding.appendChild(item);
+
+            item.onclick = () => {
+              itemSliding.open('end');
+            };
+          }, 250);
+        </script>
+      </ion-content>
+    </ion-app>
+  </body>
+</html>
diff --git a/core/src/components/item-sliding/test/async/item-sliding.e2e.ts b/core/src/components/item-sliding/test/async/item-sliding.e2e.ts
new file mode 100644
index 0000000000..ed83be79d4
--- /dev/null
+++ b/core/src/components/item-sliding/test/async/item-sliding.e2e.ts
@@ -0,0 +1,23 @@
+import { expect } from '@playwright/test';
+import { test } from '@utils/test/playwright';
+
+test.describe('item-sliding: async', () => {
+  test('should open even when item is added async', async ({ page, skip }) => {
+    skip.rtl();
+    skip.mode('md');
+
+    await page.goto(`/src/components/item-sliding/test/async`);
+
+    const itemEl = page.locator('ion-item');
+    const itemSlidingEl = page.locator('ion-item-sliding');
+
+    // Wait for item to be added to DOM
+    await page.waitForSelector('ion-item');
+
+    // Click item to open ion-item-sliding
+    await itemEl.click();
+
+    // This class is added when the item sliding component is fully open
+    await expect(itemSlidingEl).toHaveClass(/item-sliding-active-slide/);
+  });
+});
